Procedure for increased recovery of Campylobacter jejuni from inoculated unpasteurized milk

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Isolation of Campylobacter jejuni from raw milk.

Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks.

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Comparison of methods for isolating Campylobacter jejuni from raw milk.

The method of Doyle and Roman (Appl. Environ. Microbiol. 43:1343-1353, 1982) was compared with that of Lovett et al. (Appl. Environ. Microbiol. 46:459-462, 1983) for the ability to recover Campylobacter jejuni strains inoculated into raw milk at a concentration of less than 1 cell per g. The method of Lovett et al. gave significantly greater recovery proportions.

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Difficulty in recovering inoculated Campylobacter jejuni from dry poultry-associated samples.

We inoculated 5 cm2 of clean chick pads, 5 g of clean pine shavings, and fresh unsanitized broiler breeder eggshell halves with a cell suspension of Campylobacter jejuni in physiological saline. Inoculation levels were 10(2), 10(3), or 10(4) cells per sample. The samples were allowed to remain at room temperature for 15, 30, or 60 min before addition of enrichment broth. When chick pad samples ...

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Assess the prevalence rate of Campylobacter genus and Campylobacter jejuni species in raw milk collected from the Amol City by Multiplex- Polymerase Chain Reaction

Background & Objective: Campylobacter can be transmitted through the raw milk. The purpose of this study was to determine the prevalence of Campylobacter genus and Campylobacter jejuni (C. jejuni) species in raw milk samples. Materials & Methods: In this study, 72 samples of raw milk were collected of the platforms milk in the Amol city in summer. Phenotypic identification of Campylobacter genu...

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Effect of steam, lactic acid and combined treatments for inactivating Campylobacter jejuni inoculated on chicken skin

Raw poultry carcasses are often contaminated with pathogens, including Salmonella spp., Campylobacter jejuni and Listeria monocytogenes. Campylobacteriosis is an emerging and one of the most frequently reported zoonotic infectious disease. Further to the best management practices in husbandry, slaughtering and processing, decontaminating treatments might be required to control microbial hazards...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1984

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.47.3.455-460.1984